Enhancement of nucleation during hanging drop protein crystallization using HF Treatment of cover glasses
✍ Scribed by Yun-Zhu Guo; Da-Chuan Yin; Qin-Qin Lu; Xi-Kai Wang; Jun Liu
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 367 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0232-1300
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
We examined a simple approach, i.e., etching cover glasses using hydrofluoric acid (HF), to determine whether cover glass treatment enhances nucleation in hanging drop protein crystallization. Hen egg white lysozyme and proteinase K were used as the model proteins. We found that the treatment increased the success rate of crystallization. The results indicated that the simple treatment, which is easy to adopt without changing much in the hanging drop method, can be utilized as an alternative method to enhance protein crystallization screens (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)