Enhancement of nitroxide-reducing activity in rats after chronic administration of vitamin E, vitamin C, and idebenone examined by an in Vivo electron spin resonance technique
✍ Scribed by Seiji Matsumoto; Norio Mori; Nobuaki Tsuchihashi; Tateaki Ogata; Yijing Lin; Hidekatsu Yokoyama; Shin-Ichi Ishida
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 424 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0740-3194
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✦ Synopsis
Abstract
Rats were given vitamin E (vit‐E), idebenone (ID), or vitamin (C wit‐C) in their food for 2 or 4 weeks. After feeding, the ability of rats to reduce 4‐hydroxy‐2,2,6,6‐tetramethylpiperidine‐l‐oxyl (Tempol) in terms of the half‐life of Ternpol was examined as a specific marker. Ternpol was repeatedly injected intravenously, and its half‐life was serially evaluated by an in vivo electron spin resonance (ESR) technique. The radical‐reducing ability in rats was enhanced differently by Vit‐E, ID, and Vit‐C, i.e., slow onset of the ability after Vit‐E and ID (lipid‐soluble antioxidants) and fast onset after Vit‐C (a water‐soluble antioxidant).