Enhancement of crystallization with nucleotide ligands identified by dye-ligand affinity chromatography
β Scribed by Heungbok Kim, Cecelia Webster, Justin K. M. Roberts, Juthamas Kositsawat, Li-Wei Hung, Thomas C. Terwilliger, Chang-Yub Kim
- Book ID
- 113073420
- Publisher
- Springer Netherlands
- Year
- 2012
- Tongue
- English
- Weight
- 378 KB
- Volume
- 13
- Category
- Article
- ISSN
- 1345-711X
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A new method for the purification of human erythrocyte phosphoglyceratekinase involving affinity chromatography on dye-ligand media (Red A), in the presence of 3-phosphoglycerate and ATP, is described. The method is rapid and technically simple. The purity of the enzyme was verified by electrophores
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