Enhancement of buoyant separations between DNAs in preparative CsCl gradients containing distamycin A or netropsin
✍ Scribed by Kathleen M. Tatti; Michael E.S. Hudspeth; Paul H. Johnson; Lawrence I. Grossman
- Book ID
- 102985278
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 606 KB
- Volume
- 89
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
The buoyant separation in CsCl gradients between DNAs of various base composition has been studied in the presence of the polypeptide antibiotics, distamycin A and netropsin. The DNAs covered a range between 39.8 and 72% guanine + cytosine. The antibiotics, which bind preferentially to adenine-thymine-rich sequences, enhanced the separation between DNAs by an average factor of 3.5 for distamycin and 2.7 for netropsin over that found in their absence. The absolute separation for a pair of DNAs is a linear function of the base compositional difference between them.