Enhancement of bovine growth hormone gene expression by increasing the plasmid copy number
β Scribed by Jang Won Choi; Kyung Soo Ra; Young Se Lee
- Book ID
- 110225142
- Publisher
- Springer Netherlands
- Year
- 1999
- Tongue
- English
- Weight
- 67 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0141-5492
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Seven transformants having varying numbers of non-homologously integrated copies of the isocitrate lyase gene, acu-7, were analysed for enzyme activity. Maximum levels of activity, 3.8 times that of the wild type, were observed in a transformant with only two gene copies whereas eight gene copies in
## Abstract ## Background A model for __in vivo__ gene therapy based on electroporation of human growth hormone (hGH)βcoding naked DNA in the muscle of dwarf (lit/lit) and immunodeficient dwarf (lit/scid) mice is described. ## Methods A plasmid containing the ubiquitin C promoter and the genomic
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