Enhanced β-galactosidase production by high cell-density culture of recombinant Bacillus subtilis with glucose concentration control

Enhanced growth of sf-9 cells to a maxim

Enhanced growth of sf-9 cells to a maximum density of 5.2 × 107 cells per mL and production of β-galactosidase at high cell density by fed batch culture

✍ Cynthia B. Elias; Arno Zeiser; Charles Bédard; Amine A. Kamen 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 207 KB 👁 1 views

Significant improvement in cell growth and protein production has been achieved in Sf-9 insect cell cultures using pulse additions of multicomponent nutrient feed concentrates (Be ´dard et al., 1994;Chan et al., 1998). The present work focuses on investigating an alternative feeding strategy wherein