Enhanced tumor targeting by an intratumoral injection of colloidal chromic32P in two human tumors (AsPC-1 pancreas and Ls174T colon) in nude mice
✍ Scribed by Lee, Intae
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 91 KB
- Volume
- 70
- Category
- Article
- ISSN
- 0022-4790
No coin nor oath required. For personal study only.
✦ Synopsis
Background and Objectives:
To find the mechanisms of the ongoing clinical trials in intralesional colloidal chromic 32 P ( 32 P-CP) brachytherapy, the cellular uptake of 32 P-CP, changes in tumor interstitial fluid pressure (TIFP), and tumor blood flow (TBF) using two (AsPC-1, Ls174T) human tumors were measured. Methods: After exposure to 32 P-CP using exponential and plateau-phase cells, cells were trypsinized at various time intervals, then measured for the levels of radioactivity using a ␥-counter. Also measured were TIFP using the WIN technique and TBF with laser Doppler flowmetry. Results: The plateau growth-phase of both tumors showed the maximal uptake of 32 P-CP at ∼100 min. TBF decreased within 10 min after an intratumoral (i.t.) injection of 32 P-CP, and reached 75% of control value by 1 h. Conclusions: If 32 P-CP was introduced i.t., it maintained highly efficient tumor targeting, mainly due to two physiological mechanisms: the high adherence of 32 P-CP to the infused regions and the reduction in TBF by this therapeutic colloid.