Enhanced RNA expression of tissue inhibitor of metalloproteinases-1 (TIMP-1) in human breast cancer

✍ Scribed by Hitoshi Yoshiji; Daniel E. Gomez; Unnur P. Thorgeirsson

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: French
• Weight: 408 KB
• Volume: 69
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/(sici)1097-0215(19960422)69:2<131::aid-ijc11>3.0.co;2-c

✦ Synopsis

Tissue inhibitor of metalloproteinases-I (TIMP-I) is known to have at least 2 distinct types of activity, i.e., as a regulator of collagenolytic activity, and erythroid potentiating activity (EPA). In this study, we examined the expression of TIMP-I in human mammary carcinomas, non-malignant breast tissues and benign breast tumors. A total of 53 samples were subjected to Northernblot analysis, including 23 of primary breast cancer, 26 of non-malignant breast tissues, and 4 of benign tumors. Of the 53 samples, I0 were paired malignant and non-malignant breasttissue samples from the same patient. TIMP-I RNA expression was significantly higher in the malignant tumor tissues than in the non-malignant counterpart. Similar differences were ob- sewed in the level of TIMP-I protein expression in the paired breast samples examined. Moreover, breast-cancer cell lines secreted larger amounts of TIMP-I in vitro than non-neoplastic breast epithelial lineis. The up-regulation of TIMP-I expression in breast cancer may suggest that TIMP-I has an additional role to that of metalloprcrteinase inhibitor.