✦ LIBER ✦
Enhanced release of rosmarinic acid from Coleus blumei permeabilized by dimethyl sulfoxide (DMSO) while preserving cell viability and growth
✍ Scribed by Chang-Ho Park; Blanca C. Martinez
- Book ID
- 102766256
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 603 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
✦ Synopsis
Continuous permeabilization of preconditioned Coleus blurnei cells with dimethyl sulfoxide (DMSO) is shown to
be an effective strategy for the enhanced release of rosmarinic acid (RA) while preserving cell viability. When nonpreconditioned cells were permeabilized with DMSO, they lost their viability at DMSO concentrations higher than a critical value located between 0.1% and 0.5% DMSO. Product release was low I0.49 g RA/100 g dry cell weight (DCW)] at 0.1% DMSO. Preconditioning cells at 0.1% DMSO ensured high viability at DMSO concentrations of 0.5%, l.Ooh, and 1.5%. Product release reached a maximum of 2.85 g RA/ 100 g DCW at 0.5% DMSO, which was 66.4% of the total rosmarinic acid produced.