Enhanced production of γ-cyclodextrin from corn syrup solids by means of cyclododecanone as selective complexant
✍ Scribed by Jacob A. Rendleman Jr.
- Book ID
- 102992885
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 939 KB
- Volume
- 247
- Category
- Article
- ISSN
- 0008-6215
No coin nor oath required. For personal study only.
✦ Synopsis
In the presence of cyclododecanone as complexant, corn syrup solids (dextrose equivalent, d.e., 25; ap 4.5) were converted into cyclomaltooctaose (y-cyclodedrin, -y-CD) in 28% yield by incremental addition of cyclodextrin glucanotransferase (CGTase) at 60°C and pH 7.2. Cyclotridecanone was less effective in enhancing y-CD yield (14%); cyclic complexants with fewer than 12 ring atoms or more than 13 ring atoms were ineffective. In systems containing cyclododecanone, o-glucose strongly inhibited the conversion of both maltodextrin (starch hydrolyzate of ap 22) and corn syrup solids (ap 5). Maltose, when present in large proportion, also decreased yields of y-CD from corn syrup solids, but not from maltodextrin. Maltotriose had no inhibiting effect on either substrate. The nature of the high-molecular-weight fraction (ap > 10) of starch hydrolyzates was found to influence y-CD production and was the predominant factor causing yields from corn syrup solids to be lower than those from maltodextrin. Maltose itself did not undergo conversion; however, other low-molecular-weight maltooligosaccharides were converted into y-CD in good yield when treated incrementally with CGTase in the presence of cyclododecanone: 20.1% from maltotriose, 36.5% from maltotetraose, 44.1% from maltopentaose, 41.0% from maltohexaose, and 34.7% from maltoheptaose. Yields from maltooligosaccharides were adversely affected by the presence of both o-glucose and maltose.