Enhanced Production of Podophyllotoxin by Fed-batch Cultivation of Podophyllum hexandrum

✍ Scribed by Saurabh Chattopadhyay; Virendra S. Bisaria; Sant S. Bhojwani; Ashok Kumar Srivastava

• Book ID: 102806112
• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 719 KB
• Volume: 81
• Category: Article
• ISSN: 0008-4034
• DOI: 10.1002/cjce.5450810512

✦ Synopsis

Abstract

Podophyllum hexandrum (Indian May Apple) was successfully cultivated in a 3 L stirred tank bioreactor under low shear conditions in batch and fed‐batch modes of operation. A statistically optimized culture medium was used for the batch cultivation of Podophyllum hexandrum. Under optimum culture conditions of P. hexandrum, the batch culture showed a growth‐associated product formation with a maximum biomass of 21.4 g/L dry cell weight (DCW) basis and a podophyllotoxin production of 13.8 mg/L in 26 d. A mathematical model was developed to design the nutrient feeding strategies for a fed‐batch cultivation to prolong the productive log phase of cultivation. The fed‐batch cultivation was able to enhance the biomass and podophyllotoxin accumulation to 48 g/L (DCW basis) and 43.2 mg/L, respectively, in 60 d. The volumetric productivity of podophyllotoxin in fed‐batch cultivation was found to be 0.72 mg/(L. d) as opposed to 0.53 mg/(L. d) in batch cultivation under optimized culture conditions.