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Enhanced primers for amplification of DNA barcodes from a broad range of marine metazoans

✍ Scribed by Lobo, Jorge; Costa, Pedro M; Teixeira, Marcos AL; Ferreira, Maria SG; Costa, Maria H; Costa, Filipe O


Book ID
121623119
Publisher
BioMed Central
Year
2013
Tongue
English
Weight
394 KB
Volume
13
Category
Article
ISSN
1472-6785

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Cycled Primer Extension: A Method for DN
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A method has been developed for simultaneous radiolabeling and amplification of DNA hybridization probes. The method is termed cycled primer extension (CPE). CPE is a series of temperature-driven reactions in which template DNA is successively denatured and extended by a thermostable primer-dependen