Enhanced performance of fibre-optic immunoprobes using refunctionalized fluoropolymers as the substratum
✍ Scribed by Frank V. Bright; Kevin S. Litwiler; Terrance G. Vargo; Joseph A. Gardella Jr.
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 731 KB
- Volume
- 262
- Category
- Article
- ISSN
- 0003-2670
No coin nor oath required. For personal study only.
✦ Synopsis
Surface refunctlonahzed fluoropolymers are compared to quartz substrata as a means of stablhzmg covalently-lmmobdlzed blologlcal recogmtlon elements Results show that antIbodies lmmoblbzed on the refunctlonahzed fluoropolymers have greater stablhty (by at least a factor of 20) Fluoropolymer-lmmoblhzed antlbodles retain up to 85% of their mltlal affmlty after one year of refngerated (4°C) storage A new fibre-optlc lmmunoprobe, based on these refunctlonahzed fluoropolymers, IS described Comparison studies between quartz and the refunctlonahzed fluoropolymer substrata show that the detection hnnts and dynamic range are slmdar and probe stablhty IS Improved slgmficantly usmg the new materials