Enhanced parainfluenza I (6/94) virus detection in latently infected human brain cell cultures by treatment with cytochalasin D and dimethyl sulfoxide
✍ Scribed by Zofia Wroblewska; Mary Wellish; Donald H. Gilden
- Publisher
- John Wiley and Sons
- Year
- 1978
- Tongue
- English
- Weight
- 506 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0146-6615
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
The ability of cytochalasin D (CD) and dimethyl sulfoxide (DMSO) to enhance parainfluenza I (6/94) virus replication was studied in various cell culture systems. Treatment of CV~1~ cells with CD (1 μg/ml) dissolved in DMSO prior to primary 6/94 virus exposure at 10^0^–10^5^ multiplicities of infection did not substantially enhance virus replication. However, there was a transient increase in cell associated virus one day after infection of DMSO‐treated cultures. CD treatment of cultures of human brain cells latently infected with 6/94 virus (LIHB cells) did not enhance 6/94 virus detection. Cocultivation of CV~1~ cells with CD‐treated LIHB cell cultures, and cocultivation of LIHB cell cultures with CD‐treated CV~1~ cells, resulted in the production of both cell‐associated and cell‐free 6/94 virus three and five days after cocultivation. No virus was detected after similar cocultivation of untreated LIHB cell cultures with untreated CV~1~ cells. The usefulness of CD‐DMSO treatment in the rescue of virus from 6/94 LIHB cell cultures appears limited to a cocultivation system. The use of these techniques to enhance virus rescue from human tissues suspected of harboring latent viral genomes is discussed.