✦ LIBER ✦

Enhanced Imaging on Mirror Slides : Application to Cell and Tissue Fluorescence Imaging

✍ Scribed by Emmanuel Fort; Eric Le Moal; Sandrine Lévêque-Fort

Publisher: Wiley (John Wiley & Sons)
Year: 2008
Weight: 425 KB
Volume: 10
Category: Article
ISSN: 1439-4243
DOI: 10.1002/imic.200890068

No coin nor oath required. For personal study only.

✦ Synopsis

Fluorescence microscopy has become the method of choice in the majority of life-science applications. However, standard fluorophores do not luminesce brightly enough for some applications. We describe development and use of mirror slides to significantly enhance the fluorescence signal using standard air microscope objectives. This technique offers sufficient gain to achieve high-sensitivity imaging, together with a wide field of observation and a large depth of focus.

Application to Cell Imaging for Cancer Research

As an example, we show imaging improvements brought about by mirror slides in cell imaging of dog kidney cells. These are model subjects for the study of subcellular structures within epithelial cells in the context of cancer research [7].

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