Enhanced expression of P-selectin (CD62P) by endothelial cells seeded onto synthetic arterial prostheses (PET, Dacron�) is correlated with leukocyte interactions
✍ Scribed by Guidollet, Jeannine ;Chignier, Elza ;Pillot, Raymonde ;Gayet, Odile ;MacGregor, John ;Louisot, Pierre
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 175 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0021-9304
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✦ Synopsis
This study evaluated the expression by seeded endothelial cells (S-EC) of P-selectin (CD62P/GMP-140/ PADGEM), an adhesion molecule implicated in endothelialleukocyte interactions. Endothelial cells were seeded onto knitted polyethylene terephthalate (PET, Dacron) prostheses and compared with control endothelial cells (C-EC) cultured in flasks to the same stage. Using flow cytometry techniques, we observed that CD62P expression by PET S-EC was significantly increased (p < 0.05) compared to C-EC. Moreover, RT PCR techniques showed that the CD62P RNA level was higher on S-ECs compared to C-ECs. Following adhesion assays, increased polymorphonuclear neutrophil (PMN) attachment to the PET-seeded prostheses as compared to control cultures (p < 0.001) was observed. PMN adherence was enhanced by TNF␣ activation. PMN adhe-sion was decreased significantly (p < 0.001) after the incubation of resting EC or TNF␣-activated EC-seeded prostheses with a blocking monoclonal antibody (LYP20) directed against the P-selectin. Such results suggest that: (1) PET prosthetic material may induce the expression of P-selectin by S-EC; (2) seeding conditions provoke an increase in PMN adhesion; (3) increased PMN interactions with seeded PET material is partially dependent upon P-selectin expression by the S-EC.