Enhanced expression of cryIA(a) gene of Bacillus thuringiensis in Escherichia coli
โ Scribed by Varatharajalu Udayasuriyan; Akira Nakamura; Haruhiko Masaki; Takeshi Uozumi
- Book ID
- 113397939
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 482 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0922-338X
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We have cloned a 14 kb DNA segment containing the coding sequence (polC) for DNA polymerase III (PolIII) from the Bacillus subtilis chromosome. The plasmid carrying the sequence, pRO10, directs the synthesis of the 160 kDa PolIII protein and three additional polypeptides in Escherichia coli maxicell
The gene (cytA) coding for the 27 kDa polypeptide of the Bacillus thuringiensis var. israelensis mosquito larvicidal delta-endotoxin, was cloned into a plasmid containing the T7 bacteriophage promoter. The plasmid was used to transform an Escherichia coli strain containing the T7 RNA polymerase gene