Enhanced Detection of Chemiluminescence Intensity during an Oxidative Burst Reaction in Suspension Cultured Nicotiana tabacum Cells through the Use of Ficoll
✍ Scribed by Isak B. Gerber; Ian A. Dubery
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 47 KB
- Volume
- 306
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Glass petri dishes provide tight control of oxygen concentration during continuous nitrogen gassing to cultured cells. This control has led to a quick and highly reproducible simulated ischemic model of cortical neuron death in vitro. Twenty-five minutes of simulated ischemia was required for 92% death in cortical neuron cultures on glass. The contamination of leached oxygen from plastic culture vessels, in comparative experiments, necessitates longer times of simulated ischemia (120 min) to achieve similar cell death. Steady-state release of oxygen from plastic petri dishes was observed for up to 60 min of nitrogen gassing, providing a metabolic environment contrary to that of anoxia. Further investigations with models of simulated ischemia in glass culture vessels could answer some criticisms of in vitro models and their relevance to in vivo situations (10).