Enhanced cytotoxicity of core modified chitosan based polymeric micelles for doxorubicin delivery
✍ Scribed by Yi-Qing YE; Feng-Ying Chen; Qiao-ai Wu; Fu-Qiang Hu; Yong-Zhong Du; Hong Yuan; He-Yong Yu
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 373 KB
- Volume
- 98
- Category
- Article
- ISSN
- 0022-3549
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✦ Synopsis
In this study, the cytotoxicity of doxorubicin (DOX) loaded stearic acid grafted chitosan oligosaccharide (CSO-SA) micelles and its core modified drug delivery systems were investigated in vitro. The in vitro drug release experiments using cellular culture medium, Roswell Park Memorial Institute 1640 (RPMI-1640) medium as a dissolution medium confirmed that the DOX release from CSO-SA micelles was successfully delayed by the core modification of CSO-SA micelles with stearic acid (SA). The cell viability assay against A549 cells indicated the 50% inhibition concentration (IC 50 ) of blank CSO-SA micelles and the core modified CSO-SA micelles was 369 AE 27 mg/mL and 234 AE 9 mg/mL, respectively. The entrapment of DOX by CSO-SA micelles could decrease the IC 50 of DOX from 3.5 to 1.9 mg/mL, and a further reduction to 0.7 mg/mL could result by the core modification of CSO-SA micelles. The fluorescence image observations of DOX and DOX concentration measurements inside A549 cells demonstrated that the DOX concentration inside cells was increased by the entrapment of CSO-SA micelles, and further enhanced by the core modification of CSO-SA micelles. The results indicated that the CSO-SA micelles with modified cores could be useful as a drug delivery vehicle for cancer chemotherapy.