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Enhanced bone formation by marrow-derived endothelial and osteogenic cell transplantation

✍ Scribed by Sang-Soo Kim; Min Sun Park; Seung-Woo Cho; Sun Woong Kang; Kang-Min Ahn; Jong-Ho Lee; Byung-Soo Kim

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
378 KB
Volume
92A
Category
Article
ISSN
1549-3296

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✦ Synopsis

Abstract

Bone marrow‐derived osteogenic cells can regenerate bone tissues__in vivo__. The aim of the present study is to determine whether the cotransplantation of bone marrow‐derived endothelial‐like cells (BMECs) enhances bone regeneration by bone marrow‐derived osteogenic cell (BMOC) transplantation in osseous defects. Canine bone marrow cells were differentiated separately into BMECs and BMOCs. Using apatite‐coated poly(lactide‐co‐glycolide)/hydroxyapatite composite scaffolds as cell delivery vehicles, BMOCs were transplanted with or without BMECs into critical‐sized calvarial defects in immunodeficient mice. Histological analyses, microcomputed tomography, and soft X‐ray were performed to assess mineralized bone formation at 8 weeks. Cotransplantation of BMECs and BMOCs resulted in greater bone formation than transplantation of BMOCs alone. There was a significant (p < 0.05) increase in bone formation area following cotransplantation (30.8% ± 2.5%), compared with transplantation of BMOCs alone (15.3% ± 1.9%). These results demonstrate that the cotransplantation of BMECs enhances bone regeneration mediated by BMOC transplantation in osseous defects. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010

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