Enhanced bioconversion of vanillic acid into vanillin by the use of ?natural? cellobiose

✍ Scribed by Bonnin, Estelle; Lesage-Meessen, Laurence; Asther, Marcel; Thibault, Jean-Fran�ois

• Book ID: 101225107
• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 97 KB
• Volume: 79
• Category: Article
• ISSN: 0022-5142
• DOI: 10.1002/(sici)1097-0010(19990301)79:3<484::aid-jsfa271>3.0.co;2-h

✦ Synopsis

Cellulose-rich residue was obtained by a pectinase treatment of sugar beet pulp and was enzymatically degraded to produce cellobiose. From sugar beet pulp, the hydrolysis yielded 5.3% of cellobiose and 3.8% of glucose. The cellobiose-rich fraction was added in a culture medium of the ülamentous fungus Pycnoporus cinnabarinus to test it as an activator of vanillin production. Adding the sugar beet-derived cellobiose-rich fraction to a 3-day-old culture yielded a 3.3-fold increase in vanillin production when compared to a culture devoid of cellobiose, while commercial cellobiose yielded a 3.1-fold increase.