Enhanced bioanalyte detection in waveguide confined Raman spectroscopy using wavelength modulation

✍ Scribed by Praveen C. Ashok; Anna Chiara De Luca; Michael Mazilu; Kishan Dholakia

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 726 KB
• Volume: 4
• Category: Article
• ISSN: 1864-063X
• DOI: 10.1002/jbio.201000107

✦ Synopsis

Abstract

Waveguide confined Raman spectroscopy (WCRS) incorporates a fibre based Raman detection system in a microfluidic platform enabling the spectroscopic detection of analyte. It offers the possibility to develop portable, alignment free devices for bio‐analyte sensing with minimal sample preparation. Ultimate sensitivity is limited by the fibre auto‐fluorescence background. Here we report enhanced bio‐analyte detection sensitivity by combining WCRS with continuous wavelength modulation technique. We used urea as a model analyte and the modulation parameters have been optimized to maximize the sensitivity of the device. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)