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Enhanced adventive embryogenesis resulting from plasmolysis of cultured wild carrot cells

โœ Scribed by D. F. Wetherell

Book ID
104618646
Publisher
Springer
Year
1984
Tongue
English
Weight
346 KB
Volume
3
Category
Article
ISSN
0167-6857

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โœฆ Synopsis

Adventive embryogenesis in vitro-grown somatic ceils ofDaucus carota L. was increased three-fold by a 45 rain plasmolysis pre-treatment using 1M sucrose solutions. A high degree of synchronous development also resulted from this treatment. The enhancement of embryogenesis is interpreted as an increase in the regeneration of cells which have become physiologically somewhat isolated from the tissue of their origin by the plasmolysis-caused rupture of plasmodesmata. Possible causes of the increased synchrony are discussed.

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โœ William R. Krul ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› Springer ๐ŸŒ English โš– 485 KB

Roots of nine cultivars of carrot (Daucus carom L.) were exposed to one, two or three months cold treatment and cells isolated from cold-treated and control roots were assayed for the production of somatic embryos. Cells obtained from the one-or two-months cold treatments formed embryos earlier, pro