Enhanced adenoviral-vector mediated gene transfer using human albumin solder
✍ Scribed by Arnold DC Rivera; Charles N. Walker; Clifford Bleustein; Benjamin Choi; Dix P. Poppas; Diane Felsen
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 192 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0196-8092
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Background and Objective
Laser tissue welding with human albumin solder (HAS) has been used as an alternative method of wound closure. Adenoviral vectors have been used to introduce various cytokine genes into wounds to accelerate wound closure. In the present study, we were interested in the effect of HAS on adenoviral vector transfer of the β‐galactosidase (β‐gal) gene in vitro and in vivo.
Study Design/Materials and Methods
3T3 fibroblasts were used to study the effect of HAS on β‐gal gene transfer in vitro. The presence of β‐gal was determined by Western blot, and its activity by a colorimetric assay. A punch biopsy model of wound healing in pigs was used for in vivo experiments.
Results
HAS increased the efficiency of adenoviral‐mediated β‐gal transduction and stabilized the adenovirus at room temperature. HAS protected adenovirus from inactivation by laser, both in vitro and in vivo.
Conclusions
HAS may stabilize adenoviral vectors to deliver cytokine genes in future wound healing experiments. Lasers Surg. Med. 30:313–319, 2002. © 2002 Wiley‐Liss, Inc.