Preparation of a Cell-Free Translation S
โ
R. Carroll; J. Lucaslenard
๐
Article
๐
1993
๐
Elsevier Science
๐
English
โ 620 KB
Cell-free translation systems prepared from suspension-cultured HeLa S3 cells or mouse I cells by hypotonic shock followed by Dounce homogenization poorly initiated the translation of exogenous mRNA. In contrast, cell extracts prepared from cells exposed to the detergent lysolecithin translated exog