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Engineering Pichia pastoris for biocatalysis: co-production of two active enzymes

✍ Scribed by Mark S Payne; Kelly L Petrillo; John E Gavagan; Robert DiCosimo; L.Winona Wagner; David L Anton


Book ID
117522402
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
168 KB
Volume
194
Category
Article
ISSN
0378-1119

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## Abstract The methylotrophic yeast __Pichia pastoris__ has been used for the expression of many proteins, including antibody fragments. However, limitations became obvious especially when secreting heterodimeric Fab fragments. Up‐to‐date, antibody fragments have only been expressed under control