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Engineering of a fungal β-galactosidase to remove product inhibition by galactose

✍ Scribed by Xuejun Hu; Sylvain Robin; Shane O’Connell; Gary Walsh; J. Gerard Wall


Publisher
Springer
Year
2010
Tongue
English
Weight
703 KB
Volume
87
Category
Article
ISSN
1432-0614

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Significant improvement in cell growth and protein production has been achieved in Sf-9 insect cell cultures using pulse additions of multicomponent nutrient feed concentrates (Be ´dard et al., 1994;Chan et al., 1998). The present work focuses on investigating an alternative feeding strategy wherein