✦ LIBER ✦

Engineering mRNA Translation Initiation to Enhance Transient Gene Expression in Chinese Hamster Ovary Cells

✍ Scribed by Michèle F. Underhill; Clare Coley; John R. Birch; Alison Findlay; Robert Kallmeier; Christopher G. Proud; David C. James

Book ID: 109386599
Publisher: American Institute of Chemical Engineers
Year: 2003
Tongue: English
Weight: 261 KB
Volume: 19
Category: Article
ISSN: 8756-7938
DOI: 10.1021/bp025560b

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Mild Hypothermia Improves Transient Gene

Mild Hypothermia Improves Transient Gene Expression Yields Several Fold in Chinese Hamster Ovary Cells

✍ Sarah Wulhfard; Stéphanie Tissot; Sophie Bouchet; Jean Cevey; Maria de Jesus; Da 📂 Article 📅 2008 🏛 American Institute of Chemical Engineers 🌐 English ⚖ 364 KB

Enhancement of transient gene expression

Enhancement of transient gene expression and culture viability using Chinese hamster ovary cells overexpressing Bcl-xL

✍ Brian S. Majors; Michael J. Betenbaugh; Nels E. Pederson; Gisela G. Chiang 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 500 KB 👁 2 views

## Abstract Transient gene expression (TGE) provides a method for quickly delivering protein for research using mammalian cells. While high levels of recombinant proteins have been produced in TGE experiments in HEK 293 cells, TGE efforts in the commercially prominent CHO cell line still suffer fro

Insulin-induced gene 33 mRNA expression

Insulin-induced gene 33 mRNA expression in Chinese hamster ovary cells is insulin receptor dependent

✍ Peter A. Meléndez; Nicola Longo; Braulio D. Jimenez; Carmen L. Cadilla 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 182 KB 👁 2 views

Gene 33 (g33) is a non-tissue-specific gene regulated in rat liver and hepatoma cells by insulin and other agents. It is thought to participate in the transition from quiescence to proliferation in mitogen-treated cells. The mechanism(s) by which insulin exerts its action on g33 are not totally unde

Scalable transient gene expression in Ch

Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems

✍ Natalie Muller; Madiha Derouazi; Frédéric Van Tilborgh; Sarah Wulhfard; David L. 📂 Article 📅 2007 🏛 Springer Netherlands 🌐 English ⚖ 232 KB

Enhanced expression of various exogenous

Enhanced expression of various exogenous genes in recombinant Chinese hamster ovary cells in presence of dimethyl sulfoxide

✍ Chi-Hsien Liu; I-Ming Chu; Shiaw-Min Hwang 📂 Article 📅 2001 🏛 Springer Netherlands 🌐 English ⚖ 87 KB

Transient expression of human TorsinA en

Transient expression of human TorsinA enhances secretion of two functionally distinct proteins in cultured Chinese hamster ovary (CHO) cells

✍ Lyne Jossé; C. Mark Smales; Mick F. Tuite 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 491 KB 👁 2 views

Cultured mammalian cells, particularly Chinese hamster ovary (CHO) cells, are widely exploited as hosts for the production of recombinant proteins, but often yields are limiting. Such limitations may be due in part to the misfolding and subsequent degradation of the heterologous proteins. Consequent