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Engineering allosteric control to an unregulated enzyme by transfer of a regulatory domain

✍ Scribed by Cross, P. J.; Allison, T. M.; Dobson, R. C. J.; Jameson, G. B.; Parker, E. J.


Book ID
118746019
Publisher
National Academy of Sciences
Year
2013
Tongue
English
Weight
433 KB
Volume
110
Category
Article
ISSN
0027-8424

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A double mutant (R9E/M17K) of pigeon liver malic enzyme with glutamate and lysine replaced for arginine and methionine at positions 9 and 17, respectively, was found to be much more stable in urea and thermal denaturation, but was enzymatically less active than the wild-type enzyme (WT). Unfolding o