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Engineered gene over-expression as a method of drug target identification

โœ Scribed by Christopher J. Sugden; Janine R. Roper; Jeffrey G. Williams


Book ID
116290881
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
431 KB
Volume
334
Category
Article
ISSN
0006-291X

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Transient ectopic expression as a method
โœ James Witham; Susanne Vidot; Roshan Agarwal; Stanley B. Kaye; Alan Richardson ๐Ÿ“‚ Article ๐Ÿ“… 2008 ๐Ÿ› John Wiley and Sons ๐ŸŒ French โš– 204 KB

## Abstract Ectopic expression provides an alternative to RNAi to conduct functional genomic screens. We have determined whether transient expression can be used to identify genes which confer drug resistance. We constructed a bigenic vector that allows high throughput cloning and which also encode