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Energy-transfer measurements of the Cys35-Cys84 distance in bovine cardiac troponin C

✍ Scribed by Ying-Ming Liou; Franklin Fuchs


Book ID
113269035
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
632 KB
Volume
1202
Category
Article
ISSN
0167-4838

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We used time-dependent fluorescence energy transfer to determine the distribution of donorto-acceptor distances in native and denatured troponin I(Tn1). The single tryptophan residue (Trp 158) of TnI served as the donor (D), and the acceptor (A) was a labeled cysteine residue (Cys 133). The time-dep