Energy-transfer immunoassay: A study of the experimental parameters in an assay for human serum albumin

✍ Scribed by C.S. Lim; J.N. Miller; J.W. Bridges

• Publisher: Elsevier Science
• Year: 1980
• Tongue: English
• Weight: 613 KB
• Volume: 108
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(80)90709-5

✦ Synopsis

The use of fluorescein and rhodamine as fluorescent groups in energy-transfer immunoassays has been evaluated by developing an assay for human serum albumin. The sensitivity of the assay is found to depend on several factors, including the degrees of labeling of antigen and antibody, concentrations of labeled antigen and antibody, and the fluorimeter spectral bandwidth. Although fluorescein and rhodamine are far from ideal as energy-donor and -acceptor labels, the assay is capable of detecting nanomolar concentrations of albumin. Comparative studies on serum samples show that the assay gives results that compare well with those obtained using the slower electroimmunoassay method.