Energy transfer from carotenoid and FMO-protein in subcellular preparations from green sulfur bacteria. Spectroscopic characterization of an FMO-reaction center core complex at low temperature
✍ Scribed by Christof Francke; Stephan C. M. Otte; Mette Miller; Jan Amesz; John M. Olson
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 560 KB
- Volume
- 50
- Category
- Article
- ISSN
- 0166-8595
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✦ Synopsis
The Fenna-Matthews-Olson (FMO)-protein and the FMO-reaction center (RC) core complex from the green sulfur bacterium Chlorobium tepidum were examined at 6 K by absorption and fluorescence spectroscopy. The absorption spectrum of the RC core complex was obtained by a subtraction method and found to have five peaks in the Qv region, at 797, 808, 818,834 and 837 nm. The efficiency of energy transfer from carotenoid to bacteriochlorophyll a in the RC core complex was 23% at 6 K, and from the FMO-protein to the core it was 35%. Energy transfer from the FMO-protein to the core complex was also measured in isolated membranes of Prosthecochloris aestuarii from the action spectra of charge separation. Again, a low efficiency of energy transfer was obtained, both at 6 K and at room temperature.