Energy transfer: A general method for the study of protein-nucleic acid interactions
โ Scribed by Ronald P. Taylor; Richard L. Riley; Daniel Weber
- Book ID
- 115703160
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 550 KB
- Volume
- 180
- Category
- Article
- ISSN
- 0003-9861
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
## Abstract | I. | Introduction | 306 | | II. | Supramolecular Structure Characterization | 308 | | | A.โA Protein Is Used as a โHookโ | 309 | | | โโโ1.โImmunoโPrecipitation | 309 | | | โโโ2.โTagging with a PolyโHistidine Sequence | 310 | | | โโโ3.โDirect Analysis of Large Protein Complexes | 3
A rapid mixing-photocrosslinking technique has been developed to investigate the kinetics of protein-nucleic acid interactions. With this technique, binding of nucleic acid to protein is first synchronized by rapid mixing in a stopped-flow apparatus. The intermediates formed at different stages of t
An absolute method for measuring affinity constants and binding site sizes for the non-sequence-specific binding of protein to single-or double-stranded polynucleotide chains is described, based on the difference in the rate of sedimentation of free protein and the protein-nucleic acid complexes. In