Energy metabolism of reticulocytes: Two different sources of energy for Na+K+-ATPase activity

✍ Scribed by Professor Milosav. M. Kostić; R. V. Živković

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 455 KB
• Volume: 12
• Category: Article
• ISSN: 0263-6484
• DOI: 10.1002/cbf.290120205

✦ Synopsis

Abstract

Total energy production in rabbit reticulocytes amounted to 136·52 ± 6·50μmol ATP h^−1^ml^−1^ of reticulocytes: 88·3 per cent was provided by oxidative phosphorylation, whereas only 11·7 per cent by aerobic glycolysis. Na^+^K^+^‐ATPase accounted for 23 per cent, i.e. 27·65 ± 2·55μmol ATP h^−1^ml^−1^ of reticulocytes, in the overall energy consumption in reticulocytes of rabbits. Under basal conditions ATP for Na^+^K^+^‐ATPase activity was derived exclusively from oxidative phosphorylation. However, when the activity of Na^+^K^+^‐ATPase was increased due to the stimulation of adenylate cyclase by (−)‐isoprenaline, the additional energy required was provided by aerobic glycolysis. These results indicate that two different compartments, one cytosolic and the other mitochondrial, provide energy for Na^+^K^+^‐ATPase activity in reticulocytes.