- Growth of Vibrio succinogenes with nitrate as terminal electron acceptor was found to be a function of the following two catabolic reactions:
The latter reaction (b) was responsible for growth with nitrite. 2. Either succinate or fumarate could serve as sole carbon source during growth with nitrate or nitrite. Biosynthesis from succinate proceeded via fumarate. The ATP requirement for cell synthesis from succinate was equal to that calculated earlier for growth with fumarate as carbon source and electron acceptor (Brounder et al. 1982).
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The cell yield at infinite dilution rate (Ym"x) as obtained with chemostat cultures was 8.5 g dry cells/tool formate with either nitrate or nitrite as acceptor. This value amounts to 60 % of that measured earlier with fumarate as acceptor (Mell et al. 1982).
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Membrane vesicles prepared from V. succinogenes catalyzed electron transport from H 2 to nitrite. The reaction was dependent on the menaquinone present in the membrane.
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Electron transport with Hz and nitrite was coupled to the phosphorylation of ADP. The P/H2 ratio with nitrite was 40 % of that measured with fumarate as acceptor using the same preparation. The phosphorylation but not the electron transport was abolished by an uncoupling agent.