Energy biotechnology: beyond the general lignocellulose-to-ethanol pathway

Secretion-dependent proteolysis of heter

Secretion-dependent proteolysis of heterologous protein by recombinant Escherichia coli is connected to an increased activity of the energy-generating dissimilatory pathway

✍ Michael Schmidt; Elisenda Viaplana; Frank Hoffmann; Sabine Marten; Antonio Villa 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 234 KB 👁 1 views

The synthesis of a proteolytically unstable protein, originally designed for periplasmic export in recombinant Escherichia coli BL21(DE3), a strain naturally deficient for the ATP-dependent protease Lon (or La) and the outer membrane protease OmpT, is associated with a severe growth inhibition. This