Energetics of tobacco cells,Nicotiana tabacumL., growing on sucrose medium
✍ Scribed by Akira Kato; Shiro Nagai
- Book ID
- 104787273
- Publisher
- Springer
- Year
- 1979
- Tongue
- English
- Weight
- 361 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1432-0614
No coin nor oath required. For personal study only.
✦ Synopsis
Batch cultures of tobacco cells induced from Nicotiana tabacum L. cv. Bright Yellow-2 were carried out under oxygen-limited conditions using sucrose as the sole carbon source. Maintenance coefficients for sugar, m, and for oxygen, m o , were 0.02 mmol glucose/g cell dry weight/h and 0.09 mmol O2/g cell dry weight/h, and true growth yields for sugar, YG, and for oxygen, YGO, were 107 g cell dry weight/mol glucose and 61 g cell dry weight/tool 02, respectively.
Balance equations based on electrons available from the culture suggested that the carbon-substrate consumed by the cells might be metabolized mainly in biosynthetic processes without the excretion of extracellular products.