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Energetics of Heme Binding to Native and Denatured States of Cytochrome b 562†

✍ Scribed by Robinson, Clifford R.; Liu, Yufeng; Thomson, James A.; Sturtevant, Julian M.; Sligar, Stephen G.


Book ID
126861965
Publisher
American Chemical Society
Year
1997
Tongue
English
Weight
166 KB
Volume
36
Category
Article
ISSN
0006-2960

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The binding of pancreatic ribonuclease-A by denatured DNA, native DSA, poly-dA, and poly-dT, has been studied by a gel filtration method. With denatured DNA at pH 7.5, ionic strength 0.053M, there is one binding site per 12 nucleotides and the equilibrium binding constant per site is 9.7 X 10' l./rn