Endpoint markers for cancer chemoprevention trials derived from the lesion of precancer (intraepithelial neoplasia) measured by computer-assisted quantitative image analysis
✍ Scribed by Charles W. Boone; Gary J. Kelloff
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 312 KB
- Volume
- 77
- Category
- Article
- ISSN
- 0730-2312
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✦ Synopsis
Endpoint markers for cancer chemoprevention clinical trials are described that are developed from the morphological properties of the precancerous lesion of intraepithelial neoplasia itself, as measured by computer-assisted quantitative image analysis. The markers include increased proliferative fraction (percentage MIB-1 positive nuclear area); nuclear DNA content (DNA ploidy), including DNA content exceeding fivefold the haploid amount (5Cexceeding rate); nuclear/nucleolar morphometry; and disorganization of nuclear chromatin pattern as characterized by Markovian parameters and other functions. A significant new advance in image analysis is the process of ''tiling,'' in which hundreds of full monitor image fields of a given histological section at ϫ40 magnification are reduced in size and fused seamlessly to produce a single image of the histological section at ϫ1.25 magnification. The operator may review the low-power image and retrieve ϫ40 magnification of any desired area by point/clicking with a mouse.