The incidence of hypertension is increasing and it is more common in man than in women. Up to date, MMP-3 5A/6A polymorphism has been associated with artery stiffening and elevated blood pressure, whereas results considering association of endothelial NOS (eNOS) G894 T polymorphism with hypertension are controversial. The aim of our study was to analyze the possible association of eNOS G894 T and MMP-3 5A/6A gene polymorphisms with hypertension in Serbian population. Study sample consisted of 172 hypertensive and 200 normotensive subjects divided by gender. Both female and male group was truncated according to age. All subjects were genotyped for MMP-3 5A/6A and eNOS G894 T polymorphism. There was a significantly higher (Po0.05) prevalence of 5A/5A genotype in hypertensive females compared to normotensive ones (19.30 % vs. 10.84%) even more pronounced in group Z50 years, according to its recessive effect. In young males (o40 years), we found 3.7-fold increased risk for hypertension associated with allele 6A (Po0.01), and 8.1-fold with genotype 6A/6A (P 5 0.01) according to recessive model. We found no association of eNOS G894 T polymorphism with hypertension. These results indicate that there were genderand age-specific differences in association of MMP-3 5A/6A polymorphism with hypertension in Serbian population.