Endoscopic optical coherence tomography and laser-induced fluorescence spectroscopy in a murine colon cancer model
✍ Scribed by Lida P. Hariri; Alexandre R. Tumlinson; David G. Besselsen; Urs Utzinger; Eugene W. Gerner; Jennifer K. Barton
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 297 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0196-8092
No coin nor oath required. For personal study only.
✦ Synopsis
Background and objectives:
The diagnostic feasibility of optical coherence tomography (oct) and laser-induced fluorescence (lif) have been evaluated for human colorectal cancer. this study applies these technologies to a murine model of colorectal adenoma.
Study design/materials and methods:
The lower colon of 10 apc(min) and two c57bl/6j mice was surveyed over five 4-week intervals using a prototype 2.0 mm diameter oct-lif endoscope-based system. four categories were histologically classified: control c57bl/6j, adenomatous, non-diseased regions of adenomatous, and non-diseased apc(min). oct images were compared to histology. spectra from the four categories were compared via the student's t-test.
Results:
Three apc(min) and two control mice completed the study. one adenoma was histologically identified; oct visualized mucosal thickening/abnormal mass development over the imaging timepoints. lif spectral comparisons revealed decreased 405 nm intensity and the presence of a peak at 680 nm in the adenomatous apc(min).
Conclusions:
These preliminary data indicate endoscopic oct-lif has the potential to identify colorectal adenomas in murine models.