Endoluminal local delivery of PCNA/cdc2 antisense oligonucleotides by porous balloon catheter does not affect neointima formation or vessel size in the pig coronary artery model of postangioplasty restenosis
✍ Scribed by Robinson, Keith A. ;Chronos, Nicolas A.F. ;Schieffer, Elisabeth ;Palmer, Spencer J. ;Cipolla, Gustavo D. ;Milner, Peter G. ;King, Spencer B.
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 128 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0098-6569
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✦ Synopsis
Localized delivery of antisense oligonucleotides directed against cell cycle regulatory proteins has been proposed as a means to prevent restenosis after angioplasty. To test whether single endoluminal delivery of a combination of proliferating cell nuclear antigen (PCNA) and cell-division cycle 2 kinase (cdc2) antisense might affect restenosis, we delivered 2 ml of lipid-complexed PCNA/cdc2 antisense oligomers (1.35 mg) to the coronary arteries of pigs after balloon overstretch angioplasty (AS group) and performed planimetric histomorphometry on arterial sections of the tissue, harvested at 4 wk. Compared with controls receiving 38-58 reversed sequence oligomers (REV group), there were no differences in absolute intimal area (AS 1.36 6 0.08 mm 2 , REV 1.23 6 0.10 mm 2 , P 5 NS), intimal area normalized to extent of injury (AS 0.67 6 0.03, REV 0.77 6 0.10, P 5 NS), or vessel perimeter (AS 7.72 6 0.19 mm, REV 7.36 6 0.22 mm, P 5 NS). We conclude that single endoluminal delivery of antisense against key cell cycle regulatory proteins does not affect neointima formation or vessel size in this model of restenosis.