Endogenous plasma N-acetylcysteine and single dose oral bioavailability from two different formulations as determined by a new analytical method
✍ Scribed by Bernard Gabard; Hermann Mascher
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 416 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0142-2782
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✦ Synopsis
A method to quantitate N-acetylcysteine in plasma using reductive cleavage with tributylphosphine and post-HPLC column derivatisation with o-phthalaldehyde is described. Using this method, endogenous average concentrations of 0.08 jtM were measured in 10 volunteers participating in a crossover study to compare the bioavailability of two different formulations of N-acetylcysteine. The drug was detected in plasma for up to 12 h after administration of a single oral dose (200 mg); the C,,, values were up to 20 times the endogenous levels. The sensitivity and selectivity of the method should thus enable the behaviour of N-acetylcysteine after oral administration to be properly described and bioavailability studies to be performed.
KEY WORDS N-acetylcysteine Pharmacokinetics Bioavailability Endogenous level
* These results were presented in part, in a poster session at the international conference 'Detection in Liquid Chromatography and Flow Injection Analysis' (Cordoba, Spain, 1989