Recombinant Saccharomyces cerevisiae TMB3001, harboring the Pichia stipitis genes XYL1 and XYL2 (xylose reductase and xylitol dehydrogenase, respectively) and the endogenous XKS1(xylulokinase), can convert xylose to ethanol. About 30% of the consumed xylose, however, is excreted as xylitol. Enhanced
✦ LIBER ✦
Endogenous NADPH-dependent aldose reductase activity influences product formation during xylose consumption in recombinant Saccharomyces cerevisiae
✍ Scribed by K. L. Träff-Bjerre; M. Jeppsson; B. Hahn-Hägerdal; M.-F. Gorwa-Grauslund
- Book ID
- 111703620
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 138 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0749-503X
- DOI
- 10.1002/yea.1072
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To enhance metabolite transfer in the two initial sequential steps of xylose metabolism in yeast, two structural genes of Pichia stipitis, XYL } and XYL " encoding xylose reductase (XR) and xylitol dehydrogenase (XDH), respectively, were fused in frame. Four chimeric genes were constructed, encoding