Endogenous material in brain inhibiting [3H]nicotine and [3H]acetylcholine binding

✍ Scribed by Dr. H. Sershen; M. E. A. Reith; A. Hashim; A. Lajtha

• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 393 KB
• Volume: 12
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.490120405

✦ Synopsis

The supernatant obtained from mouse brain homogenates contains material that inhibits the saturable binding of [3H]nicotine in mouse cerebral cortex. This inhibitory material was further purified by heat denaturation, ultrafiltration through an Amicon PM-10 membrane filter, and gel chromatography on Sephadex G-10. The material inhibited the binding of [3HH]acetylcholine with the same potency as it did that of [3H]nicotine. It also had some affinity for the sites that specifically bind ['HID-Ala, D-Leu enkephalin, but had much lower affinity for the binding sites for [3H]quinuclidinyl benzilate (QNB), (3H]spiroperidol, [3H]naloxone, or ['Hlimipramine. Acid hydrolysis destroyed the activity. These preliminiary results suggest the presence in brain of "nicotinelike" substances, one of which may be the endogenous ligand for the sites that specifically bind [3H]ni~~tine.