Endogenous degradation of rat liver chromatin studied by agar gel electrophoresis of nuclei

✍ Scribed by Ludmila Kokileva

• Book ID: 104776843
• Publisher: Springer
• Year: 1989
• Tongue: English
• Weight: 593 KB
• Volume: 13
• Category: Article
• ISSN: 0301-4851
• DOI: 10.1007/bf00444309

✦ Synopsis

Direct agar gel electrophoresis of incubated rat liver nuclei revealed that most of the chromatin is rapidly converted to stable, large fragments, showing identical electrophoretic mobility. Short and long term incubation gave the same results. The analysis of deproteinized DNA under nondenaturing as well as denaturing conditions showed, however, a correlation between the DNA size pattern and the time of incubation. Our data on the persistance of large and uniform in size chromatin fragments despite the presence of cleaved DNA in them may indicate naturally footprinted regions of chromatin, implying most probably some strong ordered interactions of chromatin constituents. It seems that some substantial unknown features of higher order structure of chromatin are preserved in rat liver nuclei isolated and digested under the experimental conditions used.