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End-to-side anastomosis in heterotopic rat organ transplantation

✍ Scribed by Antonio Marni; Maria Elena Ferrero; Domenico Forti

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 500 KB
Volume: 17
Category: Article
ISSN: 0738-1085
DOI: 10.1002/(sici)1098-2752(1996)17:1<21::aid-micr4>3.0.co;2-z

No coin nor oath required. For personal study only.

✦ Synopsis

A partial modification of the standard end-to-side anastomo-ods. The authors thus recommend the method for performing sis usually performed in heterotopic vascularized organ heart, kidney, pancreas and small bowel transplantations in transplantation in the rat is described. Use of the method the rat. completely abolished the approximately 20% severe postdeclamping bleeding due to gaps in those blind areas, the 0 1996 Wiley-Liss, Inc.

stitching of which are at risk using previously described meth-MICROSURGERY 17:21-24 1996 vascularized organ transplantation in rats is usually performed by anastomosing in end-to-side fashion the donor arterial and venous stump to the host infraabdominal aorta and vena cava.lP4 To avoid undue manipulation of the donor organ, it is better to perform the posterior row by the endoluminal approach. This approach created some technical difficulties in our e ~p e r i e n c e . ~-~

We were able to overcome the problem with the technique described herein in detail. Wall structural differences exist between artery and vein that condition some minor modifications in the performance of the two anastomoses. The two procedures are therefore described separately.

MATERIALS AND METHODS Animals

Male Wistar adult rats weighing about 250 and 300 g were used as donors and recipients, respectively. Animals were housed and cared for in accord with our institution's guidelines for experimental animals.

Preoperative Care and Anesthesia ients. The rats were anesthetized with ether. Food and water were not restricted for donors or recip-

Experimental Design

Recipient animals were randomly allocated to two groups of 20 each, and 10 heterotopic heart and 10 hetero-From the lstituto di Patologia Generale, and Centro di Studio

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