Enantioseparation of cetirizine by sulfated-β-cyclodextrin-mediated capillary electrophoresis

✍ Scribed by Yu-Wei Chou; Wei-Shan Huang; Chen-Chun Ko; Su-Hwei Chen

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 565 KB
• Volume: 31
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200700487

✦ Synopsis

Abstract

A sulfated β‐cyclodextrin (sulfated β‐CD)‐mediated capillary electrophoresis method is described for the enantioseparation of cetirizine using achiral cefazolin as an internal standard. The enantioseparation of the drug was performed in a borate buffer (5 mM, pH 8.7) with 1% sulfated β‐CD (w/v) as chiral selector at 10 kV. Several parameters affecting the separation were studied, including the pH and the concentration of borate buffer and chiral selector. Under optimized conditions, a baseline separation of two enantiomers was achieved in less than 7 min. Using cefazolin as an internal standard (IS), the linear range of the method for the determination of levocetirizine was over 1.0 to 50.0 μg/mL; the detection limit (signal‐to‐noise ratio = 3) of levocetirizine was 0.5 μg/mL. The method allowed the enantioseparation of cetirizine in bulk samples and enantiomeric purity evaluation of levocetirizine (R‐enantiomer) in pharmaceutical tablets (Xyzal®), and it was also found to be suitable for enantioseparation in human plasma.