Enantiomeric separation of chiral polychlorinated biphenyls by micellar electrokinetic chromatography using mixtures of bile salts and sodium dodecyl sulphate with and without γ-cyclodextrin in the separation buffer

✍ Scribed by A. L. Crego; M. A. García; M. L. Marina

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 189 KB
• Volume: 12
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/(sici)1520-667x(2000)12:1<33::aid-mcs5>3.0.co;2-w

✦ Synopsis

The possibilities of using bile salts alone and mixed with sodium Ž . Ž . dodecyl sulphate SDS in micellar electrokinetic chromatography MEKC to Ž . achieve the enantiomeric separation of chiral polychlorinated biphenyls PCBs Ž have been studied. In these conditions, only three IUPAC numbers 84, 95, and . 176 of the nineteen chiral PCBs stable at room temperature were enantiomerically separated. However, up to fifteen chiral PCBs were separated, each one in its two Ž . enantiomers, when a chiral additive as ␥-cyclodextrin ␥-CD was added to the Ž . separation buffer which contained a bile salt sodium cholate, SC and SDS. The influence of ␥-CD concentration on the enantiomeric resolution of all chiral PCBs stable at room temperature was studied using buffers containing a mixed micellar system SCrSDS. Enantiomeric separations of multicomponent mixtures of chiral Ž . PCBs were also performed using a 0.1-M 2-N-cyclohexylamino -ethanesulphonic Ž . Ž . acid CHES buffer pH s 9 , 0.05-M SCr0.05-M SDS, and 0.05-M ␥-CD. Mixtures of up to eight PCBs were baseline separated into all their sixteen enantiomers in only 35 minutes. ᮊ 2000